Detailed information of TA system TA103678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3850115..3850809
• Replicon: chromosome
• Accession: NZ_CP028755
• Organism: Escherichia coli strain A20

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: AOY73_RS18780
• Protein ID: WP_001263500.1
• Coordinates: 3850115..3850513 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AOY73_RS18785
• Protein ID: WP_000554758.1
• Coordinates: 3850516..3850809 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY73_RS18755 (3845480)	3845480..3845938	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
AOY73_RS18760 (3846199)	3846199..3847656	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
AOY73_RS18765 (3847713)	3847713..3848327	-	615	WP_000602135.1	peptide chain release factor H	-
AOY73_RS18770 (3848324)	3848324..3849463	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
AOY73_RS18775 (3849653)	3849653..3850105	-	453	WP_001059897.1	GNAT family N-acetyltransferase	-
AOY73_RS18780 (3850115)	3850115..3850513	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AOY73_RS18785 (3850516)	3850516..3850809	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AOY73_RS18790 (3850861)	3850861..3851916	-	1056	WP_001552473.1	DNA polymerase IV	-
AOY73_RS18795 (3851987)	3851987..3852910	-	924	WP_001532973.1	putative lateral flagellar export/assembly protein LafU	-
AOY73_RS18800 (3852913)	3852913..3853776	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
AOY73_RS18805 (3853789)	3853789..3854505	-	717	WP_087890336.1	FliA/WhiG family RNA polymerase sigma factor	-
AOY73_RS18810 (3854525)	3854525..3854992	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T103678 WP_001263500.1 NZ_CP028755:c3850513-3850115 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T103678 NZ_CP028755:c3850513-3850115 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT103678 WP_000554758.1 NZ_CP028755:c3850809-3850516 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT103678 NZ_CP028755:c3850809-3850516 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3