Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2545952..2546505 Replicon chromosome
Accession NZ_CP028755
Organism Escherichia coli strain A20

Toxin (Protein)


Gene name higB Uniprot ID A0A0G9FUJ7
Locus tag AOY73_RS12275 Protein ID WP_000605680.1
Coordinates 2545952..2546221 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AOY73_RS12280 Protein ID WP_000781370.1
Coordinates 2546221..2546505 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY73_RS12250 (2541538) 2541538..2541969 - 432 WP_000152305.1 peroxiredoxin OsmC -
AOY73_RS12255 (2542314) 2542314..2542529 + 216 WP_000495766.1 biofilm-dependent modulation protein -
AOY73_RS12260 (2542631) 2542631..2542768 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AOY73_RS12265 (2542925) 2542925..2544622 + 1698 WP_000433462.1 malate dehydrogenase -
AOY73_RS12270 (2544756) 2544756..2545766 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
AOY73_RS12275 (2545952) 2545952..2546221 + 270 WP_000605680.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY73_RS12280 (2546221) 2546221..2546505 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
AOY73_RS12285 (2546734) 2546734..2547387 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
AOY73_RS12290 (2547380) 2547380..2548261 - 882 WP_096980409.1 formate dehydrogenase N subunit beta -
AOY73_RS12295 (2548274) 2548274..2551321 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10181.73 Da        Isoelectric Point: 6.8582

>T103675 WP_000605680.1 NZ_CP028755:2545952-2546221 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

>T103675 NZ_CP028755:2545952-2546221 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACACGATT
ACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTACATCCGTTAA
GCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAATGGCGATGCA
TACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT103675 WP_000781370.1 NZ_CP028755:2546221-2546505 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT103675 NZ_CP028755:2546221-2546505 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FUJ7

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References