Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
Location | 2545952..2546505 | Replicon | chromosome |
Accession | NZ_CP028755 | ||
Organism | Escherichia coli strain A20 |
Toxin (Protein)
Gene name | higB | Uniprot ID | A0A0G9FUJ7 |
Locus tag | AOY73_RS12275 | Protein ID | WP_000605680.1 |
Coordinates | 2545952..2546221 (+) | Length | 90 a.a. |
Antitoxin (Protein)
Gene name | higA | Uniprot ID | S1PAQ1 |
Locus tag | AOY73_RS12280 | Protein ID | WP_000781370.1 |
Coordinates | 2546221..2546505 (+) | Length | 95 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
AOY73_RS12250 (2541538) | 2541538..2541969 | - | 432 | WP_000152305.1 | peroxiredoxin OsmC | - |
AOY73_RS12255 (2542314) | 2542314..2542529 | + | 216 | WP_000495766.1 | biofilm-dependent modulation protein | - |
AOY73_RS12260 (2542631) | 2542631..2542768 | + | 138 | WP_000841554.1 | stationary-phase-induced ribosome-associated protein | - |
AOY73_RS12265 (2542925) | 2542925..2544622 | + | 1698 | WP_000433462.1 | malate dehydrogenase | - |
AOY73_RS12270 (2544756) | 2544756..2545766 | + | 1011 | WP_000642412.1 | alcohol dehydrogenase AdhP | - |
AOY73_RS12275 (2545952) | 2545952..2546221 | + | 270 | WP_000605680.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
AOY73_RS12280 (2546221) | 2546221..2546505 | + | 285 | WP_000781370.1 | HigA family addiction module antitoxin | Antitoxin |
AOY73_RS12285 (2546734) | 2546734..2547387 | - | 654 | WP_000045647.1 | formate dehydrogenase-N subunit gamma | - |
AOY73_RS12290 (2547380) | 2547380..2548261 | - | 882 | WP_096980409.1 | formate dehydrogenase N subunit beta | - |
AOY73_RS12295 (2548274) | 2548274..2551321 | - | 3048 | WP_011478186.1 | formate dehydrogenase-N subunit alpha | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 90 a.a. Molecular weight: 10181.73 Da Isoelectric Point: 6.8582
>T103675 WP_000605680.1 NZ_CP028755:2545952-2546221 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH
Download Length: 270 bp
>T103675 NZ_CP028755:2545952-2546221 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACACGATT
ACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTACATCCGTTAA
GCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAATGGCGATGCA
TACATACTGGATTACGAGGACTATCACTGA
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACACGATT
ACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTACATCCGTTAA
GCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAATGGCGATGCA
TACATACTGGATTACGAGGACTATCACTGA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10401.08 Da Isoelectric Point: 8.8567
>AT103675 WP_000781370.1 NZ_CP028755:2546221-2546505 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ
Download Length: 285 bp
>AT103675 NZ_CP028755:2546221-2546505 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A0G9FUJ7 |
Antitoxin
Source | ID | Structure |
---|---|---|
PDB | 2ICT | |
PDB | 2ICP | |
AlphaFold DB | A0A829CUG6 |