Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1959921..1960423 Replicon chromosome
Accession NZ_CP028755
Organism Escherichia coli strain A20

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag AOY73_RS09230 Protein ID WP_000767822.1
Coordinates 1960169..1960423 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AOY73_RS09225 Protein ID WP_001259255.1
Coordinates 1959921..1960172 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY73_RS09200 (1955099) 1955099..1956166 - 1068 WP_000080127.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AOY73_RS09205 (1956166) 1956166..1957236 - 1071 WP_000109002.1 histidinol-phosphate transaminase -
AOY73_RS09210 (1957233) 1957233..1958537 - 1305 WP_001445483.1 histidinol dehydrogenase -
AOY73_RS09215 (1958543) 1958543..1959442 - 900 WP_000131779.1 ATP phosphoribosyltransferase -
AOY73_RS09220 (1959588) 1959588..1959638 - 51 WP_001364200.1 his operon leader peptide -
AOY73_RS09225 (1959921) 1959921..1960172 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AOY73_RS09230 (1960169) 1960169..1960423 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AOY73_RS09235 (1960506) 1960506..1961330 + 825 WP_000754737.1 SDR family oxidoreductase -
AOY73_RS09240 (1961376) 1961376..1962305 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
AOY73_RS09245 (1962520) 1962520..1962582 + 63 WP_010723108.1 membrane protein YoeI -
AOY73_RS09250 (1962572) 1962572..1963930 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T103666 WP_000767822.1 NZ_CP028755:1960169-1960423 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T103666 NZ_CP028755:1960169-1960423 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT103666 WP_001259255.1 NZ_CP028755:1959921-1960172 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT103666 NZ_CP028755:1959921-1960172 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References