Detailed information of TA system TA103665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1245146..1245873
• Replicon: chromosome
• Accession: NZ_CP028755
• Organism: Escherichia coli strain A20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: AOY73_RS06015
• Protein ID: WP_000547563.1
• Coordinates: 1245146..1245457 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY73_RS06020
• Protein ID: WP_000126304.1
• Coordinates: 1245454..1245873 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY73_RS05985 (1240287)	1240287..1241996	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
AOY73_RS05990 (1242006)	1242006..1242548	+	543	WP_000493799.1	formate hydrogenlyase subunit HycF	-
AOY73_RS05995 (1242548)	1242548..1243315	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
AOY73_RS06000 (1243312)	1243312..1243722	+	411	WP_001291913.1	formate hydrogenlyase assembly protein HycH	-
AOY73_RS06005 (1243715)	1243715..1244185	+	471	WP_042024242.1	hydrogenase maturation peptidase HycI	-
AOY73_RS06010 (1244228)	1244228..1244983	+	756	WP_024262088.1	hypothetical protein	-
AOY73_RS06015 (1245146)	1245146..1245457	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
AOY73_RS06020 (1245454)	1245454..1245873	+	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
AOY73_RS06025 (1245965)	1245965..1246393	-	429	WP_000536064.1	hypothetical protein	-
AOY73_RS06030 (1246778)	1246778..1247305	+	528	WP_001078777.1	electron transport protein HydN	-
AOY73_RS06035 (1247458)	1247458..1249710	+	2253	WP_060588771.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T103665 WP_000547563.1 NZ_CP028755:1245146-1245457 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T103665 NZ_CP028755:1245146-1245457 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGCAAATATCCTAACGATGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT103665 WP_000126304.1 NZ_CP028755:1245454-1245873 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT103665 NZ_CP028755:1245454-1245873 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22