Detailed information of TA system TA103661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 672298..673097
• Replicon: chromosome
• Accession: NZ_CP028755
• Organism: Escherichia coli strain A20

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: AOY73_RS03300
• Protein ID: WP_000347273.1
• Coordinates: 672298..672762 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: AOY73_RS03305
• Protein ID: WP_001308975.1
• Coordinates: 672762..673097 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY73_RS03270 (667299)	667299..667733	-	435	WP_000948837.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
AOY73_RS03275 (667751)	667751..668629	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
AOY73_RS03280 (668619)	668619..669398	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
AOY73_RS03285 (669409)	669409..669882	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
AOY73_RS03290 (669905)	669905..671185	-	1281	WP_096979982.1	tagatose-bisphosphate aldolase subunit KbaZ	-
AOY73_RS03295 (671434)	671434..672243	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
AOY73_RS03300 (672298)	672298..672762	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
AOY73_RS03305 (672762)	672762..673097	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
AOY73_RS03310 (673246)	673246..674817	-	1572	WP_001273763.1	galactarate dehydratase	-
AOY73_RS03315 (675192)	675192..676526	+	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
AOY73_RS03320 (676542)	676542..677312	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T103661 WP_000347273.1 NZ_CP028755:c672762-672298 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T103661 NZ_CP028755:c672762-672298 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT103661 WP_001308975.1 NZ_CP028755:c673097-672762 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT103661 NZ_CP028755:c673097-672762 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08