Detailed information of TA system TA103655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2885335..2885837
• Replicon: chromosome
• Accession: NZ_CP028754
• Organism: Escherichia coli strain A21

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: AOY74_RS14110
• Protein ID: WP_000767819.1
• Coordinates: 2885335..2885589 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: AOY74_RS14115
• Protein ID: WP_001259253.1
• Coordinates: 2885586..2885837 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY74_RS14085 (2881153)	2881153..2881611	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
AOY74_RS14090 (2881828)	2881828..2883186	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
AOY74_RS14095 (2883176)	2883176..2883238	-	63	WP_010723108.1	membrane protein YoeI	-
AOY74_RS14100 (2883453)	2883453..2884382	-	930	WP_201482140.1	LysR substrate-binding domain-containing protein	-
AOY74_RS14105 (2884428)	2884428..2885252	-	825	WP_000754733.1	SDR family oxidoreductase	-
AOY74_RS14110 (2885335)	2885335..2885589	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
AOY74_RS14115 (2885586)	2885586..2885837	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
AOY74_RS14120 (2886120)	2886120..2886170	+	51	WP_001364200.1	his operon leader peptide	-
AOY74_RS14125 (2886316)	2886316..2887215	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
AOY74_RS14130 (2887221)	2887221..2888525	+	1305	WP_001524971.1	histidinol dehydrogenase	-
AOY74_RS14135 (2888522)	2888522..2889592	+	1071	WP_001524973.1	histidinol-phosphate transaminase	-
AOY74_RS14140 (2889592)	2889592..2890659	+	1068	WP_000080128.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2881153..2881611	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T103655 WP_000767819.1 NZ_CP028754:c2885589-2885335 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T103655 NZ_CP028754:c2885589-2885335 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT103655 WP_001259253.1 NZ_CP028754:c2885837-2885586 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT103655 NZ_CP028754:c2885837-2885586 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3