Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2340253..2340815 Replicon chromosome
Accession NZ_CP028754
Organism Escherichia coli strain A21

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag AOY74_RS11305 Protein ID WP_000605675.1
Coordinates 2340537..2340815 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AOY74_RS11300 Protein ID WP_000781370.1
Coordinates 2340253..2340537 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY74_RS11285 (2335435) 2335435..2338482 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
AOY74_RS11290 (2338495) 2338495..2339379 + 885 WP_201482151.1 formate dehydrogenase N subunit beta -
AOY74_RS11295 (2339372) 2339372..2340025 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
AOY74_RS11300 (2340253) 2340253..2340537 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
AOY74_RS11305 (2340537) 2340537..2340815 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY74_RS11310 (2341001) 2341001..2342011 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
AOY74_RS11315 (2342145) 2342145..2343842 - 1698 WP_001607334.1 malate dehydrogenase -
AOY74_RS11320 (2343998) 2343998..2344135 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AOY74_RS11325 (2344237) 2344237..2344452 - 216 WP_000495774.1 biofilm-dependent modulation protein -
AOY74_RS11330 (2344796) 2344796..2345227 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T103644 WP_000605675.1 NZ_CP028754:c2340815-2340537 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T103644 NZ_CP028754:c2340815-2340537 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT103644 WP_000781370.1 NZ_CP028754:c2340537-2340253 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT103644 NZ_CP028754:c2340537-2340253 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References