Detailed information of TA system TA103640

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2204772..2205143
• Replicon: chromosome
• Accession: NZ_CP028754
• Organism: Escherichia coli strain A21

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: AOY74_RS10635
• Protein ID: WP_001317028.1
• Coordinates: 2204949..2205143 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2204772..2204950 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY74_RS10605 (2200525)	2200525..2200698	+	174	WP_001296046.1	protein YnaL	-
AOY74_RS10610 (2200728)	2200728..2202101	+	1374	WP_022296461.1	ATP-dependent RNA helicase DbpA	-
AOY74_RS10615 (2202229)	2202229..2203164	-	936	WP_001157378.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
AOY74_RS10620 (2203216)	2203216..2204451	-	1236	WP_000040839.1	site-specific integrase	-
AOY74_RS10625 (2204453)	2204453..2204668	-	216	WP_000079604.1	excisionase XisR	-
- (2204772)	2204772..2204950	+	179	NuclAT_0	-	Antitoxin
- (2204772)	2204772..2204950	+	179	NuclAT_0	-	Antitoxin
- (2204772)	2204772..2204950	+	179	NuclAT_0	-	Antitoxin
- (2204772)	2204772..2204950	+	179	NuclAT_0	-	Antitoxin
AOY74_RS10630 (2204768)	2204768..2204956	-	189	WP_001595656.1	DUF1187 family protein	-
AOY74_RS10635 (2204949)	2204949..2205143	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
AOY74_RS10640 (2205200)	2205200..2206009	-	810	WP_001595658.1	recombination protein RecT	-
AOY74_RS10645 (2206002)	2206002..2208602	-	2601	WP_001595659.1	exodeoxyribonuclease VIII	-
AOY74_RS10650 (2208704)	2208704..2208978	-	275	Protein_2063	hypothetical protein	-
AOY74_RS10655 (2209053)	2209053..2209223	-	171	WP_001516462.1	YdaE family protein	-
AOY74_RS10660 (2209223)	2209223..2209444	-	222	WP_000560211.1	killing protein KilR	-
AOY74_RS10665 (2209855)	2209855..2210007	-	153	WP_001169150.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2203216..2245791	42575

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T103640 WP_001317028.1 NZ_CP028754:c2205143-2204949 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T103640 NZ_CP028754:c2205143-2204949 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT103640 NZ_CP028754:2204772-2204950 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTAGTTAGTTTATATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTAGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin