Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1084537..1085231 Replicon chromosome
Accession NZ_CP028754
Organism Escherichia coli strain A21

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag AOY74_RS05005 Protein ID WP_001263500.1
Coordinates 1084833..1085231 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag AOY74_RS05000 Protein ID WP_000554758.1
Coordinates 1084537..1084830 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY74_RS04980 (1080277) 1080277..1080774 + 498 WP_000006239.1 REP-associated tyrosine transposase RayT -
AOY74_RS04985 (1080890) 1080890..1082602 - 1713 Protein_954 flagellar biosynthesis protein FlhA -
AOY74_RS04990 (1082574) 1082574..1083359 + 786 WP_000207551.1 putative lateral flagellar export/assembly protein LafU -
AOY74_RS04995 (1083430) 1083430..1084485 + 1056 WP_001226168.1 DNA polymerase IV -
AOY74_RS05000 (1084537) 1084537..1084830 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
AOY74_RS05005 (1084833) 1084833..1085231 + 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
AOY74_RS05010 (1085241) 1085241..1085693 + 453 WP_071589393.1 GNAT family N-acetyltransferase -
AOY74_RS05015 (1085883) 1085883..1087022 + 1140 WP_001606885.1 RNA ligase RtcB family protein -
AOY74_RS05020 (1087019) 1087019..1087633 + 615 WP_000602123.1 peptide chain release factor H -
AOY74_RS05025 (1087689) 1087689..1089146 - 1458 WP_001293022.1 cytosol nonspecific dipeptidase -
AOY74_RS05030 (1089407) 1089407..1089865 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T103633 WP_001263500.1 NZ_CP028754:1084833-1085231 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T103633 NZ_CP028754:1084833-1085231 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT103633 WP_000554758.1 NZ_CP028754:1084537-1084830 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT103633 NZ_CP028754:1084537-1084830 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References