Detailed information of TA system TA103628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 173777..174379
• Replicon: chromosome
• Accession: NZ_CP028754
• Organism: Escherichia coli strain A21

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3L4YH82
• Locus tag: AOY74_RS00845
• Protein ID: WP_000897303.1
• Coordinates: 173777..174088 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY74_RS00850
• Protein ID: WP_000356397.1
• Coordinates: 174089..174379 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY74_RS00820 (169691)	169691..170290	+	600	WP_001296610.1	glucose-1-phosphatase	-
AOY74_RS00825 (170284)	170284..171156	+	873	WP_000920763.1	virulence factor BrkB family protein	-
AOY74_RS00830 (171153)	171153..171590	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY74_RS00835 (171635)	171635..172576	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY74_RS00840 (172640)	172640..173548	-	909	WP_001385591.1	alpha/beta hydrolase	-
AOY74_RS00845 (173777)	173777..174088	+	312	WP_000897303.1	hypothetical protein	Toxin
AOY74_RS00850 (174089)	174089..174379	+	291	WP_000356397.1	NadS family protein	Antitoxin
AOY74_RS00855 (174738)	174738..175016	+	279	WP_001296612.1	hypothetical protein	-
AOY74_RS00860 (175415)	175415..175633	+	219	WP_001251293.1	CopG family transcriptional regulator	-
AOY74_RS00865 (175818)	175818..176558	-	741	WP_022296249.1	hypothetical protein	-
AOY74_RS00870 (176583)	176583..177431	-	849	WP_001038651.1	hypothetical protein	-
AOY74_RS00875 (177721)	177721..177963	+	243	WP_001068514.1	CopG family transcriptional regulator	-
AOY74_RS00880 (178145)	178145..179074	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12302.33 Da
• Isoelectric Point: 10.0233

>T103628 WP_000897303.1 NZ_CP028754:173777-174088 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGRLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103628 NZ_CP028754:173777-174088 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTAGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAGGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103628 WP_000356397.1 NZ_CP028754:174089-174379 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103628 NZ_CP028754:174089-174379 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A3L4YH82