Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3595535..3596229 Replicon chromosome
Accession NZ_CP028753
Organism Escherichia coli strain A22

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag AOY75_RS17370 Protein ID WP_001263501.1
Coordinates 3595535..3595933 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag AOY75_RS17375 Protein ID WP_000554755.1
Coordinates 3595936..3596229 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY75_RS17345 (3590900) 3590900..3591358 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
AOY75_RS17350 (3591619) 3591619..3593076 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
AOY75_RS17355 (3593133) 3593133..3593747 - 615 WP_000602123.1 peptide chain release factor H -
AOY75_RS17360 (3593744) 3593744..3594883 - 1140 WP_000521578.1 RNA ligase RtcB family protein -
AOY75_RS17365 (3595073) 3595073..3595525 - 453 WP_072017721.1 GNAT family N-acetyltransferase -
AOY75_RS17370 (3595535) 3595535..3595933 - 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
AOY75_RS17375 (3595936) 3595936..3596229 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
AOY75_RS17380 (3596281) 3596281..3597336 - 1056 WP_001226170.1 DNA polymerase IV -
AOY75_RS17385 (3597407) 3597407..3598330 - 924 WP_001232551.1 putative lateral flagellar export/assembly protein LafU -
AOY75_RS17390 (3598333) 3598333..3599196 - 864 WP_001169519.1 flagellar motor stator protein MotA -
AOY75_RS17395 (3599209) 3599209..3599928 - 720 WP_000938737.1 FliA/WhiG family RNA polymerase sigma factor -
AOY75_RS17400 (3599948) 3599948..3600415 - 468 WP_000725264.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T103618 WP_001263501.1 NZ_CP028753:c3595933-3595535 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T103618 NZ_CP028753:c3595933-3595535 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT103618 WP_000554755.1 NZ_CP028753:c3596229-3595936 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT103618 NZ_CP028753:c3596229-3595936 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References