Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3608487..3609250 | Replicon | chromosome |
| Accession | NZ_CP028752 | ||
| Organism | Escherichia coli strain A23 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | - |
| Locus tag | AOY76_RS17625 | Protein ID | WP_240770798.1 |
| Coordinates | 3608487..3608954 (-) | Length | 156 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1FJN6 |
| Locus tag | AOY76_RS17630 | Protein ID | WP_000554757.1 |
| Coordinates | 3608957..3609250 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (3603859) | 3603859..3603939 | - | 81 | NuclAT_11 | - | - |
| - (3603859) | 3603859..3603939 | - | 81 | NuclAT_11 | - | - |
| - (3603859) | 3603859..3603939 | - | 81 | NuclAT_11 | - | - |
| - (3603859) | 3603859..3603939 | - | 81 | NuclAT_11 | - | - |
| AOY76_RS17600 (3604535) | 3604535..3604993 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| AOY76_RS17605 (3605254) | 3605254..3606711 | + | 1458 | WP_001293003.1 | cytosol nonspecific dipeptidase | - |
| AOY76_RS17610 (3606768) | 3606768..3607289 | - | 522 | Protein_3463 | peptide chain release factor H | - |
| AOY76_RS17615 (3607288) | 3607288..3607491 | - | 204 | Protein_3464 | RtcB family protein | - |
| AOY76_RS17620 (3607855) | 3607855..3608552 | + | 698 | WP_103215986.1 | IS1-like element IS1A family transposase | - |
| AOY76_RS17625 (3608487) | 3608487..3608954 | - | 468 | WP_240770798.1 | type II toxin-antitoxin system YafO family toxin | Toxin |
| AOY76_RS17630 (3608957) | 3608957..3609250 | - | 294 | WP_000554757.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| AOY76_RS17635 (3609302) | 3609302..3610357 | - | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| AOY76_RS17640 (3610428) | 3610428..3611351 | - | 924 | WP_240770800.1 | putative lateral flagellar export/assembly protein LafU | - |
| AOY76_RS17645 (3611354) | 3611354..3612217 | - | 864 | WP_001169518.1 | flagellar motor stator protein MotA | - |
| AOY76_RS17650 (3612309) | 3612309..3613006 | + | 698 | WP_103215986.1 | IS1-like element IS1A family transposase | - |
| AOY76_RS17655 (3613008) | 3613008..3613295 | + | 288 | WP_240770802.1 | type II toxin-antitoxin system mRNA interferase toxin YafQ | - |
| AOY76_RS17660 (3613451) | 3613451..3614191 | + | 741 | WP_001225679.1 | peptidoglycan meso-diaminopimelic acid protein amidase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 3571610..3609250 | 37640 | |
| - | inside | IScluster/Tn | - | - | 3608049..3613006 | 4957 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 156 a.a. Molecular weight: 18132.87 Da Isoelectric Point: 6.7564
>T103600 WP_240770798.1 NZ_CP028752:c3608954-3608487 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRGNDSNLLIVFYVQIMPDDFVMQLHRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRGNDSNLLIVFYVQIMPDDFVMQLHRF
Download Length: 468 bp
>T103600 NZ_CP028752:c3608954-3608487 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCGGTAATGACTCCA
ACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCGGTAATGACTCCA
ACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.81 Da Isoelectric Point: 5.9050
>AT103600 WP_000554757.1 NZ_CP028752:c3609250-3608957 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT103600 NZ_CP028752:c3609250-3608957 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|