Detailed information of TA system TA103581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4489635..4490434
• Replicon: chromosome
• Accession: NZ_CP028751
• Organism: Escherichia coli strain A24

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: AOY77_RS21605
• Protein ID: WP_000347252.1
• Coordinates: 4489970..4490434 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: AOY77_RS21600
• Protein ID: WP_001296435.1
• Coordinates: 4489635..4489970 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY77_RS21585 (4485420)	4485420..4486190	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
AOY77_RS21590 (4486206)	4486206..4487540	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
AOY77_RS21595 (4487915)	4487915..4489486	+	1572	WP_001273939.1	galactarate dehydratase	-
AOY77_RS21600 (4489635)	4489635..4489970	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
AOY77_RS21605 (4489970)	4489970..4490434	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
AOY77_RS21610 (4490489)	4490489..4491298	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
AOY77_RS21615 (4491547)	4491547..4492827	+	1281	WP_000681930.1	tagatose-bisphosphate aldolase subunit KbaZ	-
AOY77_RS21620 (4492850)	4492850..4493323	+	474	WP_001298322.1	PTS N-acetylgalactosamine transporter subunit IIB	-
AOY77_RS21625 (4493334)	4493334..4494113	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
AOY77_RS21630 (4494103)	4494103..4494981	+	879	WP_021552915.1	PTS N-acetylgalactosamine transporter subunit IID	-
AOY77_RS21635 (4494999)	4494999..4495433	+	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T103581 WP_000347252.1 NZ_CP028751:4489970-4490434 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T103581 NZ_CP028751:4489970-4490434 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT103581 WP_001296435.1 NZ_CP028751:4489635-4489970 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT103581 NZ_CP028751:4489635-4489970 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5