Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2525135..2525697 Replicon chromosome
Accession NZ_CP028751
Organism Escherichia coli strain A24

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag AOY77_RS12425 Protein ID WP_000605675.1
Coordinates 2525419..2525697 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AOY77_RS12420 Protein ID WP_000781370.1
Coordinates 2525135..2525419 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY77_RS12405 (2520316) 2520316..2523363 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
AOY77_RS12410 (2523376) 2523376..2524260 + 885 WP_001240581.1 formate dehydrogenase N subunit beta -
AOY77_RS12415 (2524253) 2524253..2524906 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
AOY77_RS12420 (2525135) 2525135..2525419 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
AOY77_RS12425 (2525419) 2525419..2525697 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY77_RS12430 (2525883) 2525883..2526893 - 1011 WP_000642380.1 alcohol dehydrogenase AdhP -
AOY77_RS12435 (2527027) 2527027..2528724 - 1698 WP_000433462.1 malate dehydrogenase -
AOY77_RS12440 (2528881) 2528881..2529018 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AOY77_RS12445 (2529120) 2529120..2529335 - 216 WP_000495766.1 biofilm-dependent modulation protein -
AOY77_RS12450 (2529680) 2529680..2530111 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T103566 WP_000605675.1 NZ_CP028751:c2525697-2525419 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T103566 NZ_CP028751:c2525697-2525419 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT103566 WP_000781370.1 NZ_CP028751:c2525419-2525135 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT103566 NZ_CP028751:c2525419-2525135 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References