Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1544084..1544763 Replicon chromosome
Accession NZ_CP028751
Organism Escherichia coli strain A24

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag AOY77_RS07460 Protein ID WP_000057523.1
Coordinates 1544084..1544386 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag AOY77_RS07465 Protein ID WP_000806442.1
Coordinates 1544422..1544763 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY77_RS07435 (1539460) 1539460..1541112 + 1653 WP_000771760.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
AOY77_RS07440 (1541150) 1541150..1541653 - 504 WP_000667000.1 hypothetical protein -
AOY77_RS07445 (1541650) 1541650..1542450 - 801 WP_000439798.1 hypothetical protein -
AOY77_RS07450 (1542474) 1542474..1542953 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
AOY77_RS07455 (1543157) 1543157..1543951 - 795 WP_000365148.1 TraB/GumN family protein -
AOY77_RS07460 (1544084) 1544084..1544386 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY77_RS07465 (1544422) 1544422..1544763 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
AOY77_RS07470 (1544821) 1544821..1547325 - 2505 WP_201472066.1 copper-exporting P-type ATPase CopA -
AOY77_RS07475 (1547587) 1547587..1548519 + 933 WP_000883025.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T103565 WP_000057523.1 NZ_CP028751:1544084-1544386 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T103565 NZ_CP028751:1544084-1544386 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT103565 WP_000806442.1 NZ_CP028751:1544422-1544763 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT103565 NZ_CP028751:1544422-1544763 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References