Detailed information of TA system TA103562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1245348..1246042
• Replicon: chromosome
• Accession: NZ_CP028751
• Organism: Escherichia coli strain A24

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: AOY77_RS06065
• Protein ID: WP_001263500.1
• Coordinates: 1245644..1246042 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AOY77_RS06060
• Protein ID: WP_000554758.1
• Coordinates: 1245348..1245641 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY77_RS06035 (1240884)	1240884..1241381	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
AOY77_RS06040 (1241464)	1241464..1241673	-	210	WP_011579064.1	hypothetical protein	-
AOY77_RS06045 (1241701)	1241701..1243413	-	1713	Protein_1163	flagellar biosynthesis protein FlhA	-
AOY77_RS06050 (1243385)	1243385..1244170	+	786	WP_000207578.1	putative lateral flagellar export/assembly protein LafU	-
AOY77_RS06055 (1244241)	1244241..1245296	+	1056	WP_001226168.1	DNA polymerase IV	-
AOY77_RS06060 (1245348)	1245348..1245641	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AOY77_RS06065 (1245644)	1245644..1246042	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AOY77_RS06070 (1246052)	1246052..1246504	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
AOY77_RS06075 (1246694)	1246694..1247833	+	1140	WP_000521561.1	RNA ligase RtcB family protein	-
AOY77_RS06080 (1247830)	1247830..1248444	+	615	WP_000602124.1	peptide chain release factor H	-
AOY77_RS06085 (1248501)	1248501..1249958	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
AOY77_RS06090 (1250219)	1250219..1250677	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	1225824..1246504	20680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T103562 WP_001263500.1 NZ_CP028751:1245644-1246042 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T103562 NZ_CP028751:1245644-1246042 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT103562 WP_000554758.1 NZ_CP028751:1245348-1245641 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT103562 NZ_CP028751:1245348-1245641 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3