Detailed information of TA system TA103554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 172506..173108
• Replicon: chromosome
• Accession: NZ_CP028751
• Organism: Escherichia coli strain A24

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY77_RS00835
• Protein ID: WP_000897305.1
• Coordinates: 172506..172817 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY77_RS00840
• Protein ID: WP_000356397.1
• Coordinates: 172818..173108 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY77_RS00810 (168420)	168420..169019	+	600	WP_001298594.1	glucose-1-phosphatase	-
AOY77_RS00815 (169013)	169013..169885	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY77_RS00820 (169882)	169882..170319	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY77_RS00825 (170364)	170364..171305	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY77_RS00830 (171369)	171369..172277	-	909	WP_001298596.1	alpha/beta hydrolase	-
AOY77_RS00835 (172506)	172506..172817	+	312	WP_000897305.1	hypothetical protein	Toxin
AOY77_RS00840 (172818)	172818..173108	+	291	WP_000356397.1	NadS family protein	Antitoxin
AOY77_RS00845 (173713)	173713..173931	+	219	WP_001298592.1	CopG family transcriptional regulator	-
AOY77_RS00850 (174146)	174146..175075	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
AOY77_RS00855 (175072)	175072..175707	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY77_RS00860 (175704)	175704..176606	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103554 WP_000897305.1 NZ_CP028751:172506-172817 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103554 NZ_CP028751:172506-172817 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103554 WP_000356397.1 NZ_CP028751:172818-173108 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103554 NZ_CP028751:172818-173108 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7