Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 4335697..4337015 Replicon chromosome
Accession NZ_CP028750
Organism Escherichia coli strain A25

Toxin (Protein)


Gene name HipT Uniprot ID B7UL96
Locus tag AOY78_RS21170 Protein ID WP_001262465.1
Coordinates 4335697..4336704 (-) Length 336 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID B7UL97
Locus tag AOY78_RS21175 Protein ID WP_001346664.1
Coordinates 4336704..4337015 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY78_RS21145 (4331390) 4331390..4331581 + 192 WP_000988310.1 cellulose biosynthesis protein BcsF -
AOY78_RS21150 (4331578) 4331578..4333257 + 1680 WP_000191555.1 cellulose biosynthesis protein BcsG -
AOY78_RS21155 (4333343) 4333343..4333450 - 108 WP_001295224.1 type I toxin-antitoxin system toxin Ldr family protein -
- (4333499) 4333499..4333564 + 66 NuclAT_18 - -
- (4333499) 4333499..4333564 + 66 NuclAT_18 - -
- (4333499) 4333499..4333564 + 66 NuclAT_18 - -
- (4333499) 4333499..4333564 + 66 NuclAT_18 - -
- (4333499) 4333499..4333564 + 66 NuclAT_24 - -
- (4333499) 4333499..4333564 + 66 NuclAT_24 - -
- (4333499) 4333499..4333564 + 66 NuclAT_24 - -
- (4333499) 4333499..4333564 + 66 NuclAT_24 - -
AOY78_RS21160 (4333826) 4333826..4333933 - 108 WP_000170747.1 type I toxin-antitoxin system toxin Ldr family protein -
- (4333982) 4333982..4334047 + 66 NuclAT_19 - -
- (4333982) 4333982..4334047 + 66 NuclAT_19 - -
- (4333982) 4333982..4334047 + 66 NuclAT_19 - -
- (4333982) 4333982..4334047 + 66 NuclAT_19 - -
- (4333982) 4333982..4334047 + 66 NuclAT_25 - -
- (4333982) 4333982..4334047 + 66 NuclAT_25 - -
- (4333982) 4333982..4334047 + 66 NuclAT_25 - -
- (4333982) 4333982..4334047 + 66 NuclAT_25 - -
AOY78_RS21165 (4334409) 4334409..4335680 + 1272 WP_001339917.1 aromatic amino acid transport family protein -
AOY78_RS21170 (4335697) 4335697..4336704 - 1008 WP_001262465.1 HipA domain-containing protein Toxin
AOY78_RS21175 (4336704) 4336704..4337015 - 312 WP_001346664.1 HipA N-terminal domain-containing protein Antitoxin
AOY78_RS21180 (4337000) 4337000..4337323 - 324 WP_000563102.1 helix-turn-helix transcriptional regulator -
AOY78_RS21185 (4337548) 4337548..4338561 - 1014 WP_000103582.1 dipeptide ABC transporter ATP-binding subunit DppF -
AOY78_RS21190 (4338558) 4338558..4339541 - 984 WP_001196477.1 dipeptide ABC transporter ATP-binding protein -
AOY78_RS21195 (4339552) 4339552..4340454 - 903 WP_000084677.1 dipeptide ABC transporter permease DppC -
AOY78_RS21200 (4340464) 4340464..4341483 - 1020 WP_000938855.1 dipeptide ABC transporter permease DppB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(56-302)

Antitoxin

HipA, N-terminal subdomain 1

(6-99)


Sequences

Toxin        


Download         Length: 336 a.a.        Molecular weight: 38329.55 Da        Isoelectric Point: 5.4135

>T103553 WP_001262465.1 NZ_CP028750:c4336704-4335697 [Escherichia coli]
MANCRILLTPLNERDEQRGYSTQGLKRLSGTAKLNPRLGFTRTQFVQELPRQQKGMSISGYQPKLQLVLDEGEFRVVDHQ
GNFILKPSPADFPGLAENEHATMTLMSRLGFDVPVHGLLSFAPQSEEELEYAFVIRRYDRDNKGLPVHQEQLDGAMQITD
KYGKTGNDNEQYVSYETLARFLVAHVNDNIAFKIDLFRRIVYAWLLGNNDMHLRNFGLVYSDGLTPALAPVYDFVSVAPY
PEYFYSNYLALPLLTREEGGRELAPGFHSDYGEYIGQDFLLLGESMGLAPRLLEKLFQDIRKENAIVMETYEQSFMTQDH
IQAVLQCYRHRLGLL

Download         Length: 1008 bp

>T103553 NZ_CP028750:c4336704-4335697 [Escherichia coli]
ATGGCGAATTGTCGTATTCTGTTAACGCCGTTGAATGAACGTGACGAACAGCGCGGATATTCGACACAGGGGTTGAAGCG
ACTGAGCGGCACTGCAAAATTGAATCCTCGGCTGGGGTTTACGCGAACTCAGTTTGTGCAGGAACTTCCGCGTCAACAAA
AAGGGATGAGTATTTCCGGTTACCAGCCCAAATTGCAATTAGTGCTGGATGAAGGAGAGTTTCGCGTCGTAGATCATCAG
GGAAATTTTATCCTTAAGCCCTCTCCAGCCGACTTTCCCGGTTTGGCCGAAAATGAACACGCAACAATGACATTGATGTC
TCGTTTGGGATTTGACGTACCTGTGCATGGCCTGTTGAGTTTTGCACCACAATCAGAGGAAGAGCTTGAATACGCATTTG
TTATCCGGCGCTATGACCGGGATAACAAGGGGCTGCCAGTGCATCAGGAACAGCTTGATGGCGCGATGCAGATTACAGAT
AAATATGGCAAAACGGGGAATGATAACGAACAATATGTCAGCTATGAAACGCTGGCCCGATTTTTAGTTGCCCATGTTAA
CGACAACATCGCATTTAAAATCGATCTCTTTCGTCGCATTGTTTACGCCTGGTTGCTGGGTAATAACGACATGCATTTAC
GAAACTTTGGCTTAGTGTACTCCGATGGTTTGACGCCTGCGTTAGCGCCGGTGTATGACTTTGTTTCTGTCGCTCCCTAT
CCGGAATATTTTTACTCAAACTATCTGGCGCTGCCGTTATTGACGCGAGAAGAAGGCGGTCGGGAACTGGCTCCTGGATT
TCACAGTGATTATGGCGAGTATATCGGGCAGGACTTTTTGCTGTTGGGTGAATCAATGGGGTTAGCGCCCAGATTGCTGG
AAAAACTCTTTCAGGATATACGTAAAGAAAATGCCATTGTTATGGAAACGTATGAGCAGTCCTTTATGACTCAGGATCAT
ATCCAGGCTGTGCTGCAATGCTATCGACATCGTCTGGGGCTGCTGTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11831.41 Da        Isoelectric Point: 6.3215

>AT103553 WP_001346664.1 NZ_CP028750:c4337015-4336704 [Escherichia coli]
MHRRVKVLLYGQVVGELSQNDSGFLFQYAHDYHGPAISISLPVAQRQFPSETLHPYFASLAPEGWLRQRYSQIQHRDEND
LLGMLIDNGKNLLGAIQILPWEE

Download         Length: 312 bp

>AT103553 NZ_CP028750:c4337015-4336704 [Escherichia coli]
ATGCATCGGCGAGTGAAAGTGTTGCTCTATGGTCAGGTAGTGGGTGAACTCTCTCAGAACGACAGTGGTTTTTTGTTCCA
GTACGCCCACGATTACCACGGACCGGCAATCTCAATCAGTCTGCCCGTCGCACAGCGTCAGTTTCCCAGTGAGACGTTGC
ATCCTTATTTCGCCTCACTGGCGCCAGAAGGATGGCTGCGTCAACGCTACAGCCAGATACAGCATCGTGACGAAAACGAT
CTGCTGGGAATGCTTATTGATAACGGCAAGAATCTGCTGGGGGCCATCCAGATTTTACCTTGGGAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AB4
PDB 7AB5
PDB 7AB3

Antitoxin

Source ID Structure
PDB 7AB4
PDB 7AB5
PDB 7AB3
AlphaFold DB A0A0K5V9L6

References