Detailed information of TA system TA103537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2229731..2230293
• Replicon: chromosome
• Accession: NZ_CP028750
• Organism: Escherichia coli strain A25

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A786YE86
• Locus tag: AOY78_RS10915
• Protein ID: WP_000605674.1
• Coordinates: 2230015..2230293 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY78_RS10910
• Protein ID: WP_000781364.1
• Coordinates: 2229731..2230015 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY78_RS10895 (2225090)	2225090..2228137	+	3048	WP_077767045.1	formate dehydrogenase-N subunit alpha	-
AOY78_RS10900 (2228150)	2228150..2229034	+	885	WP_001240584.1	formate dehydrogenase N subunit beta	-
AOY78_RS10905 (2229027)	2229027..2229680	+	654	WP_000045647.1	formate dehydrogenase-N subunit gamma	-
AOY78_RS10910 (2229731)	2229731..2230015	-	285	WP_000781364.1	HigA family addiction module antitoxin	Antitoxin
AOY78_RS10915 (2230015)	2230015..2230293	-	279	WP_000605674.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOY78_RS10920 (2230479)	2230479..2231489	-	1011	WP_052912873.1	alcohol dehydrogenase AdhP	-
AOY78_RS10925 (2231623)	2231623..2233320	-	1698	WP_000433462.1	malate dehydrogenase	-
AOY78_RS10930 (2233476)	2233476..2233613	-	138	WP_000841554.1	stationary-phase-induced ribosome-associated protein	-
AOY78_RS10935 (2233715)	2233715..2233930	-	216	WP_000495766.1	biofilm-dependent modulation protein	-
AOY78_RS10940 (2234275)	2234275..2234706	+	432	WP_000152307.1	peroxiredoxin OsmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10609.23 Da
• Isoelectric Point: 6.8588

>T103537 WP_000605674.1 NZ_CP028750:c2230293-2230015 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDEIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T103537 NZ_CP028750:c2230293-2230015 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGAAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10386.11 Da
• Isoelectric Point: 8.8567

>AT103537 WP_000781364.1 NZ_CP028750:c2230015-2229731 [Escherichia coli]
MKMANHPRPGDIILESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Nucleotide

• Length: 285 bp

>AT103537 NZ_CP028750:c2230015-2229731 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCTGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: A0A786YE86