Detailed information of TA system TA103525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4568976..4569578
• Replicon: chromosome
• Accession: NZ_CP028749
• Organism: Escherichia coli strain A26

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AOY79_RS22090
• Protein ID: WP_000897302.1
• Coordinates: 4569267..4569578 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY79_RS22085
• Protein ID: WP_000356397.1
• Coordinates: 4568976..4569266 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY79_RS22060 (4565049)	4565049..4565951	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
AOY79_RS22065 (4565948)	4565948..4566583	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY79_RS22070 (4566580)	4566580..4567509	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
AOY79_RS22075 (4567725)	4567725..4567943	-	219	WP_001314326.1	CopG family transcriptional regulator	-
AOY79_RS22080 (4568339)	4568339..4568617	-	279	WP_001296612.1	hypothetical protein	-
AOY79_RS22085 (4568976)	4568976..4569266	-	291	WP_000356397.1	NadS family protein	Antitoxin
AOY79_RS22090 (4569267)	4569267..4569578	-	312	WP_000897302.1	hypothetical protein	Toxin
AOY79_RS22095 (4569807)	4569807..4570715	+	909	WP_001331553.1	alpha/beta hydrolase	-
AOY79_RS22100 (4570779)	4570779..4571720	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY79_RS22105 (4571765)	4571765..4572202	-	438	WP_000560978.1	D-aminoacyl-tRNA deacylase	-
AOY79_RS22110 (4572199)	4572199..4573071	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY79_RS22115 (4573065)	4573065..4573664	-	600	WP_001314324.1	glucose-1-phosphatase	-
AOY79_RS22120 (4573763)	4573763..4574548	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T103525 WP_000897302.1 NZ_CP028749:c4569578-4569267 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103525 NZ_CP028749:c4569578-4569267 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103525 WP_000356397.1 NZ_CP028749:c4569266-4568976 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103525 NZ_CP028749:c4569266-4568976 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416