Detailed information of TA system TA103520

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-rdlD/SymE(toxin)
• Location: 4044527..4044939
• Replicon: chromosome
• Accession: NZ_CP028749
• Organism: Escherichia coli strain A26

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: AOY79_RS19715
• Protein ID: WP_000132614.1
• Coordinates: 4044598..4044939 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	4044527..4044603 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY79_RS19705 (4041139)	4041139..4042608	+	1470	WP_001387312.1	type I restriction-modification system subunit M	-
AOY79_RS19710 (4042608)	4042608..4044377	+	1770	WP_001513535.1	restriction endonuclease subunit S	-
- (4044527)	4044527..4044603	-	77	NuclAT_12	-	Antitoxin
- (4044527)	4044527..4044603	-	77	NuclAT_12	-	Antitoxin
- (4044527)	4044527..4044603	-	77	NuclAT_12	-	Antitoxin
- (4044527)	4044527..4044603	-	77	NuclAT_12	-	Antitoxin
- (4044527)	4044527..4044603	-	77	NuclAT_13	-	Antitoxin
- (4044527)	4044527..4044603	-	77	NuclAT_13	-	Antitoxin
- (4044527)	4044527..4044603	-	77	NuclAT_13	-	Antitoxin
- (4044527)	4044527..4044603	-	77	NuclAT_13	-	Antitoxin
AOY79_RS19715 (4044598)	4044598..4044939	+	342	WP_000132614.1	endoribonuclease SymE	Toxin
AOY79_RS19720 (4044986)	4044986..4046149	-	1164	WP_224153787.1	DUF1524 domain-containing protein	-
AOY79_RS19725 (4046203)	4046203..4047079	-	877	Protein_3869	DUF262 domain-containing protein	-
AOY79_RS19730 (4047485)	4047485..4048405	-	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
AOY79_RS19735 (4048590)	4048590..4049870	+	1281	WP_001338077.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	4028915..4044939	16024

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T103520 WP_000132614.1 NZ_CP028749:4044598-4044939 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T103520 NZ_CP028749:4044598-4044939 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT103520 NZ_CP028749:c4044603-4044527 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin