Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2433846..2434399 Replicon chromosome
Accession NZ_CP028749
Organism Escherichia coli strain A26

Toxin (Protein)


Gene name higB Uniprot ID A0A0G9FUJ7
Locus tag AOY79_RS11750 Protein ID WP_000605680.1
Coordinates 2433846..2434115 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AOY79_RS11755 Protein ID WP_000781370.1
Coordinates 2434115..2434399 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY79_RS11725 (2429432) 2429432..2429863 - 432 WP_000152307.1 peroxiredoxin OsmC -
AOY79_RS11730 (2430208) 2430208..2430423 + 216 WP_000495766.1 biofilm-dependent modulation protein -
AOY79_RS11735 (2430525) 2430525..2430662 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AOY79_RS11740 (2430819) 2430819..2432516 + 1698 WP_000433460.1 malate dehydrogenase -
AOY79_RS11745 (2432650) 2432650..2433660 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
AOY79_RS11750 (2433846) 2433846..2434115 + 270 WP_000605680.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY79_RS11755 (2434115) 2434115..2434399 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
AOY79_RS11760 (2434628) 2434628..2435281 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
AOY79_RS11765 (2435274) 2435274..2436158 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
AOY79_RS11770 (2436171) 2436171..2439218 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10181.73 Da        Isoelectric Point: 6.8582

>T103518 WP_000605680.1 NZ_CP028749:2433846-2434115 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

>T103518 NZ_CP028749:2433846-2434115 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACACGATT
ACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTACATCCGTTAA
GCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAATGGCGATGCA
TACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT103518 WP_000781370.1 NZ_CP028749:2434115-2434399 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT103518 NZ_CP028749:2434115-2434399 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FUJ7

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References