Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1115592..1116319 Replicon chromosome
Accession NZ_CP028749
Organism Escherichia coli strain A26

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag AOY79_RS05355 Protein ID WP_016233494.1
Coordinates 1115592..1115903 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag AOY79_RS05360 Protein ID WP_000126294.1
Coordinates 1115900..1116319 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY79_RS05330 (1111528) 1111528..1113237 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
AOY79_RS05335 (1113247) 1113247..1113789 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
AOY79_RS05340 (1113789) 1113789..1114556 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
AOY79_RS05345 (1114553) 1114553..1114963 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
AOY79_RS05350 (1114956) 1114956..1115426 + 471 WP_000132959.1 hydrogenase maturation peptidase HycI -
AOY79_RS05355 (1115592) 1115592..1115903 + 312 WP_016233494.1 type II toxin-antitoxin system HigB family toxin Toxin
AOY79_RS05360 (1115900) 1115900..1116319 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
AOY79_RS05365 (1116411) 1116411..1116839 - 429 WP_000536064.1 hypothetical protein -
AOY79_RS05370 (1117224) 1117224..1117751 + 528 WP_001078777.1 electron transport protein HydN -
AOY79_RS05375 (1117904) 1117904..1120156 + 2253 WP_001350159.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12453.25 Da        Isoelectric Point: 9.7075

>T103507 WP_016233494.1 NZ_CP028749:1115592-1115903 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNKLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T103507 NZ_CP028749:1115592-1115903 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAAGCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT103507 WP_000126294.1 NZ_CP028749:1115900-1116319 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT103507 NZ_CP028749:1115900-1116319 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGATACTAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References