Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 981104..981645 Replicon chromosome
Accession NZ_CP028748
Organism Escherichia coli strain A27

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag AOY80_RS04600 Protein ID WP_000615979.1
Coordinates 981104..981382 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AOY80_RS04605 Protein ID WP_000729704.1
Coordinates 981385..981645 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY80_RS04585 (978683) 978683..979261 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AOY80_RS04590 (979470) 979470..980237 + 768 WP_000333380.1 class II glutamine amidotransferase -
AOY80_RS04595 (980208) 980208..980948 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY80_RS04600 (981104) 981104..981382 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY80_RS04605 (981385) 981385..981645 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY80_RS04610 (981831) 981831..982604 + 774 WP_122990173.1 C40 family peptidase -
AOY80_RS04615 (982661) 982661..983017 - 357 WP_047402977.1 type II toxin-antitoxin system HicB family antitoxin -
AOY80_RS04620 (983096) 983096..984818 - 1723 Protein_885 flagellar biosynthesis protein FlhA -
AOY80_RS04625 (984790) 984790..985575 + 786 WP_000207555.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - rhs/PAAR / gmhA/lpcA 967340..983404 16064


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T103485 WP_000615979.1 NZ_CP028748:c981382-981104 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103485 NZ_CP028748:c981382-981104 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT103485 WP_000729704.1 NZ_CP028748:c981645-981385 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103485 NZ_CP028748:c981645-981385 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References