Detailed information of TA system TA103482

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4776174..4776776
• Replicon: chromosome
• Accession: NZ_CP028747
• Organism: Escherichia coli strain A28

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY81_RS23180
• Protein ID: WP_000897305.1
• Coordinates: 4776465..4776776 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY81_RS23175
• Protein ID: WP_000356397.1
• Coordinates: 4776174..4776464 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY81_RS23150 (4772100)	4772100..4773002	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
AOY81_RS23155 (4772999)	4772999..4773634	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY81_RS23160 (4773631)	4773631..4774560	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AOY81_RS23165 (4774890)	4774890..4775132	-	243	WP_001086388.1	protein YiiF	-
AOY81_RS23170 (4775351)	4775351..4775569	-	219	WP_001297075.1	CopG family transcriptional regulator	-
AOY81_RS23175 (4776174)	4776174..4776464	-	291	WP_000356397.1	NadS family protein	Antitoxin
AOY81_RS23180 (4776465)	4776465..4776776	-	312	WP_000897305.1	hypothetical protein	Toxin
AOY81_RS23185 (4777005)	4777005..4777913	+	909	WP_001162704.1	alpha/beta hydrolase	-
AOY81_RS23190 (4777977)	4777977..4778918	-	942	WP_001300148.1	fatty acid biosynthesis protein FabY	-
AOY81_RS23195 (4778963)	4778963..4779400	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY81_RS23200 (4779397)	4779397..4780269	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY81_RS23205 (4780263)	4780263..4780862	-	600	WP_001295269.1	glucose-1-phosphatase	-
AOY81_RS23210 (4780961)	4780961..4781746	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103482 WP_000897305.1 NZ_CP028747:c4776776-4776465 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103482 NZ_CP028747:c4776776-4776465 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103482 WP_000356397.1 NZ_CP028747:c4776464-4776174 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103482 NZ_CP028747:c4776464-4776174 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7