Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3866811..3867352 Replicon chromosome
Accession NZ_CP028747
Organism Escherichia coli strain A28

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag AOY81_RS18965 Protein ID WP_000615979.1
Coordinates 3867074..3867352 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AOY81_RS18960 Protein ID WP_000729704.1
Coordinates 3866811..3867071 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY81_RS18935 (3862594) 3862594..3863379 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
AOY81_RS18940 (3863351) 3863351..3865063 + 1713 Protein_3721 flagellar biosynthesis protein FlhA -
AOY81_RS18945 (3865168) 3865168..3865446 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AOY81_RS18950 (3865439) 3865439..3865795 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
AOY81_RS18955 (3865852) 3865852..3866625 - 774 WP_000543899.1 C40 family peptidase -
AOY81_RS18960 (3866811) 3866811..3867071 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY81_RS18965 (3867074) 3867074..3867352 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY81_RS18970 (3867508) 3867508..3868248 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY81_RS18975 (3868219) 3868219..3868986 - 768 WP_000333380.1 class II glutamine amidotransferase -
AOY81_RS18980 (3869192) 3869192..3869770 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T103479 WP_000615979.1 NZ_CP028747:3867074-3867352 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103479 NZ_CP028747:3867074-3867352 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT103479 WP_000729704.1 NZ_CP028747:3866811-3867071 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103479 NZ_CP028747:3866811-3867071 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References