103460

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4232058..4232555 Replicon chromosome
Accession NZ_CP028746
Organism Escherichia coli strain A29

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY82_RS20435 Protein ID WP_001173343.1
Coordinates 4232058..4232231 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag AOY82_RS20440 Protein ID WP_001425728.1
Coordinates 4232259..4232555 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY82_RS20425 (4228728) 4228728..4230185 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
AOY82_RS20430 (4230422) 4230422..4231939 + 1518 WP_001295074.1 lysine--tRNA ligase -
AOY82_RS20435 (4232058) 4232058..4232231 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY82_RS20440 (4232259) 4232259..4232555 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY82_RS20445 (4232782) 4232782..4233054 - 273 WP_086812958.1 GNAT family N-acetyltransferase -
AOY82_RS20450 (4233066) 4233066..4233296 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
AOY82_RS20455 (4233477) 4233477..4235108 + 1632 WP_001216477.1 sensor histidine kinase -
AOY82_RS20460 (4235105) 4235105..4235824 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4223687..4232555 8868


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T103460 WP_001173343.1 NZ_CP028746:c4232231-4232058 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T103460 NZ_CP028746:c4232231-4232058 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT103460 WP_001425728.1 NZ_CP028746:c4232555-4232259 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT103460 NZ_CP028746:c4232555-4232259 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References