Detailed information of TA system TA103445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1118859..1119586
• Replicon: chromosome
• Accession: NZ_CP028746
• Organism: Escherichia coli strain A29

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: AOY82_RS05440
• Protein ID: WP_000547564.1
• Coordinates: 1118859..1119170 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY82_RS05445
• Protein ID: WP_000126294.1
• Coordinates: 1119167..1119586 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY82_RS05410 (1114001)	1114001..1115710	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
AOY82_RS05415 (1115720)	1115720..1116262	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
AOY82_RS05420 (1116262)	1116262..1117029	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
AOY82_RS05425 (1117026)	1117026..1117436	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
AOY82_RS05430 (1117429)	1117429..1117899	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
AOY82_RS05435 (1117924)	1117924..1118685	+	762	WP_053275247.1	hypothetical protein	-
AOY82_RS05440 (1118859)	1118859..1119170	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
AOY82_RS05445 (1119167)	1119167..1119586	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
AOY82_RS05450 (1119700)	1119700..1121124	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
AOY82_RS05455 (1121133)	1121133..1122590	-	1458	WP_001107882.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
AOY82_RS05460 (1122850)	1122850..1123860	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
AOY82_RS05465 (1124009)	1124009..1124536	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T103445 WP_000547564.1 NZ_CP028746:1118859-1119170 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T103445 NZ_CP028746:1118859-1119170 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT103445 WP_000126294.1 NZ_CP028746:1119167-1119586 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT103445 NZ_CP028746:1119167-1119586 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5