Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3890739..3891280 Replicon chromosome
Accession NZ_CP028745
Organism Escherichia coli strain A30

Toxin (Protein)


Gene name yafQ Uniprot ID A0A246NT41
Locus tag AOY83_RS18490 Protein ID WP_000615977.1
Coordinates 3891002..3891280 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag AOY83_RS18485 Protein ID WP_000729709.1
Coordinates 3890739..3890999 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY83_RS18465 (3886515) 3886515..3887300 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
AOY83_RS18470 (3887272) 3887272..3888984 + 1713 Protein_3632 flagellar biosynthesis protein FlhA -
AOY83_RS18475 (3889107) 3889107..3889604 - 498 WP_240767410.1 REP-associated tyrosine transposase RayT -
AOY83_RS18480 (3889780) 3889780..3890529 - 750 WP_047625031.1 C40 family peptidase -
AOY83_RS18485 (3890739) 3890739..3890999 + 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY83_RS18490 (3891002) 3891002..3891280 + 279 WP_000615977.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY83_RS18495 (3891436) 3891436..3892176 + 741 WP_240767411.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY83_RS18500 (3892147) 3892147..3892914 - 768 WP_000333380.1 class II glutamine amidotransferase -
AOY83_RS18505 (3893120) 3893120..3893698 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / gmhA/lpcA 3835766..3908801 73035


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10842.67 Da        Isoelectric Point: 10.0702

>T103434 WP_000615977.1 NZ_CP028745:3891002-3891280 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNILPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103434 NZ_CP028745:3891002-3891280 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATATTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT103434 WP_000729709.1 NZ_CP028745:3890739-3890999 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103434 NZ_CP028745:3890739-3890999 [Escherichia coli]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A246NT41

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References