Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2523464..2524026 Replicon chromosome
Accession NZ_CP028745
Organism Escherichia coli strain A30

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag AOY83_RS12035 Protein ID WP_000605676.1
Coordinates 2523464..2523742 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AOY83_RS12040 Protein ID WP_000781370.1
Coordinates 2523742..2524026 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY83_RS12010 (2519050) 2519050..2519481 - 432 WP_000152305.1 peroxiredoxin OsmC -
AOY83_RS12015 (2519826) 2519826..2520041 + 216 WP_000495766.1 biofilm-dependent modulation protein -
AOY83_RS12020 (2520143) 2520143..2520280 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AOY83_RS12025 (2520437) 2520437..2522134 + 1698 WP_000433464.1 malate dehydrogenase -
AOY83_RS12030 (2522268) 2522268..2523278 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
AOY83_RS12035 (2523464) 2523464..2523742 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY83_RS12040 (2523742) 2523742..2524026 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
AOY83_RS12045 (2524255) 2524255..2524908 - 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
AOY83_RS12050 (2524901) 2524901..2525785 - 885 WP_001240582.1 formate dehydrogenase N subunit beta -
AOY83_RS12055 (2525798) 2525798..2528845 - 3048 WP_240767159.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T103427 WP_000605676.1 NZ_CP028745:2523464-2523742 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T103427 NZ_CP028745:2523464-2523742 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT103427 WP_000781370.1 NZ_CP028745:2523742-2524026 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT103427 NZ_CP028745:2523742-2524026 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCTCCCTCAACGGCAAGCCGATTGCTGACAGGAAAAGCAGCTTTGACCCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References