Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 731209..731936 Replicon chromosome
Accession NZ_CP028745
Organism Escherichia coli strain A30

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag AOY83_RS03560 Protein ID WP_000550189.1
Coordinates 731209..731523 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag AOY83_RS03565 Protein ID WP_001514564.1
Coordinates 731520..731936 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY83_RS03540 (727366) 727366..728352 - 987 WP_240767664.1 Gfo/Idh/MocA family oxidoreductase -
AOY83_RS03545 (728431) 728431..729123 - 693 WP_033815002.1 vancomycin high temperature exclusion protein -
AOY83_RS03550 (729200) 729200..729703 - 504 WP_001295542.1 M48 family metallopeptidase -
AOY83_RS03555 (729788) 729788..730924 + 1137 WP_240767665.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
AOY83_RS03560 (731209) 731209..731523 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
AOY83_RS03565 (731520) 731520..731936 + 417 WP_001514564.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
AOY83_RS03570 (731981) 731981..733999 - 2019 WP_240767666.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
AOY83_RS03575 (734303) 734303..735847 - 1545 WP_218074492.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-136)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T103413 WP_000550189.1 NZ_CP028745:731209-731523 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T103413 NZ_CP028745:731209-731523 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15025.44 Da        Isoelectric Point: 4.4547

>AT103413 WP_001514564.1 NZ_CP028745:731520-731936 [Escherichia coli]
MITIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT103413 NZ_CP028745:731520-731936 [Escherichia coli]
ATGATTACGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References