Detailed information of TA system TA103376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 156196..156798
• Replicon: chromosome
• Accession: NZ_CP028744
• Organism: Escherichia coli strain A31

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY84_RS00755
• Protein ID: WP_000897305.1
• Coordinates: 156196..156507 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY84_RS00760
• Protein ID: WP_000356397.1
• Coordinates: 156508..156798 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY84_RS00725 (151226)	151226..152011	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
AOY84_RS00730 (152110)	152110..152709	+	600	WP_001295269.1	glucose-1-phosphatase	-
AOY84_RS00735 (152703)	152703..153575	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY84_RS00740 (153572)	153572..154009	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY84_RS00745 (154054)	154054..154995	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY84_RS00750 (155059)	155059..155967	-	909	WP_001162704.1	alpha/beta hydrolase	-
AOY84_RS00755 (156196)	156196..156507	+	312	WP_000897305.1	hypothetical protein	Toxin
AOY84_RS00760 (156508)	156508..156798	+	291	WP_000356397.1	NadS family protein	Antitoxin
AOY84_RS00765 (157403)	157403..157621	+	219	WP_001315930.1	CopG family transcriptional regulator	-
AOY84_RS00770 (157841)	157841..158083	+	243	WP_001087409.1	protein YiiF	-
AOY84_RS00775 (158413)	158413..159342	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AOY84_RS00780 (159339)	159339..159974	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY84_RS00785 (159971)	159971..160873	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103376 WP_000897305.1 NZ_CP028744:156196-156507 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103376 NZ_CP028744:156196-156507 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103376 WP_000356397.1 NZ_CP028744:156508-156798 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103376 NZ_CP028744:156508-156798 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7