Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2337513..2338075 Replicon chromosome
Accession NZ_CP028743
Organism Escherichia coli strain A32

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag AOY85_RS11400 Protein ID WP_000605675.1
Coordinates 2337797..2338075 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AOY85_RS11395 Protein ID WP_000781370.1
Coordinates 2337513..2337797 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY85_RS11380 (2332694) 2332694..2335741 + 3048 WP_015912497.1 formate dehydrogenase-N subunit alpha -
AOY85_RS11385 (2335754) 2335754..2336638 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
AOY85_RS11390 (2336631) 2336631..2337284 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
AOY85_RS11395 (2337513) 2337513..2337797 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
AOY85_RS11400 (2337797) 2337797..2338075 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY85_RS11405 (2338261) 2338261..2339271 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
AOY85_RS11410 (2339405) 2339405..2341102 - 1698 WP_000433462.1 malate dehydrogenase -
AOY85_RS11415 (2341259) 2341259..2341396 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AOY85_RS11420 (2341498) 2341498..2341713 - 216 WP_000495766.1 biofilm-dependent modulation protein -
AOY85_RS11425 (2342058) 2342058..2342489 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T103361 WP_000605675.1 NZ_CP028743:c2338075-2337797 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T103361 NZ_CP028743:c2338075-2337797 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT103361 WP_000781370.1 NZ_CP028743:c2337797-2337513 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT103361 NZ_CP028743:c2337797-2337513 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References