Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1131279..1131973 Replicon chromosome
Accession NZ_CP028743
Organism Escherichia coli strain A32

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag AOY85_RS05390 Protein ID WP_001263501.1
Coordinates 1131575..1131973 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag AOY85_RS05385 Protein ID WP_000554755.1
Coordinates 1131279..1131572 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY85_RS05360 (1127093) 1127093..1127560 + 468 WP_000725264.1 flagellar basal body-associated FliL family protein -
AOY85_RS05365 (1127580) 1127580..1128299 + 720 WP_000938737.1 FliA/WhiG family RNA polymerase sigma factor -
AOY85_RS05370 (1128312) 1128312..1129175 + 864 WP_001169519.1 flagellar motor stator protein MotA -
AOY85_RS05375 (1129178) 1129178..1130101 + 924 WP_001232551.1 putative lateral flagellar export/assembly protein LafU -
AOY85_RS05380 (1130172) 1130172..1131227 + 1056 WP_001226170.1 DNA polymerase IV -
AOY85_RS05385 (1131279) 1131279..1131572 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
AOY85_RS05390 (1131575) 1131575..1131973 + 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
AOY85_RS05395 (1131983) 1131983..1132435 + 453 WP_072017721.1 GNAT family N-acetyltransferase -
AOY85_RS05400 (1132625) 1132625..1133764 + 1140 WP_000521578.1 RNA ligase RtcB family protein -
AOY85_RS05405 (1133761) 1133761..1134375 + 615 WP_000602123.1 peptide chain release factor H -
AOY85_RS05410 (1134432) 1134432..1135889 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
AOY85_RS05415 (1136150) 1136150..1136608 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T103359 WP_001263501.1 NZ_CP028743:1131575-1131973 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T103359 NZ_CP028743:1131575-1131973 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT103359 WP_000554755.1 NZ_CP028743:1131279-1131572 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT103359 NZ_CP028743:1131279-1131572 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References