Detailed information of TA system TA103352

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 688026..688438
• Replicon: chromosome
• Accession: NZ_CP028743
• Organism: Escherichia coli strain A32

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: AOY85_RS03175
• Protein ID: WP_000132614.1
• Coordinates: 688026..688367 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	688362..688438 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY85_RS03150 (683095)	683095..684375	-	1281	WP_033549583.1	DUF445 domain-containing protein	-
AOY85_RS03155 (684560)	684560..685480	+	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
AOY85_RS03160 (685708)	685708..685788	+	81	WP_020233658.1	hypothetical protein	-
AOY85_RS03165 (685886)	685886..686768	+	883	Protein_600	DUF262 domain-containing protein	-
AOY85_RS03170 (686816)	686816..687979	+	1164	WP_072002093.1	DUF1524 domain-containing protein	-
AOY85_RS03175 (688026)	688026..688367	-	342	WP_000132614.1	endoribonuclease SymE	Toxin
- (688362)	688362..688438	+	77	NuclAT_6	-	Antitoxin
- (688362)	688362..688438	+	77	NuclAT_6	-	Antitoxin
- (688362)	688362..688438	+	77	NuclAT_6	-	Antitoxin
- (688362)	688362..688438	+	77	NuclAT_6	-	Antitoxin
- (688362)	688362..688438	+	77	NuclAT_7	-	Antitoxin
- (688362)	688362..688438	+	77	NuclAT_7	-	Antitoxin
- (688362)	688362..688438	+	77	NuclAT_7	-	Antitoxin
- (688362)	688362..688438	+	77	NuclAT_7	-	Antitoxin
AOY85_RS03180 (688588)	688588..690294	-	1707	WP_001100194.1	restriction endonuclease subunit S	-
AOY85_RS03185 (690294)	690294..691763	-	1470	WP_001540814.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T103352 WP_000132614.1 NZ_CP028743:c688367-688026 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T103352 NZ_CP028743:c688367-688026 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT103352 NZ_CP028743:688362-688438 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin