Detailed information of TA system TA103350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 175762..176364
• Replicon: chromosome
• Accession: NZ_CP028743
• Organism: Escherichia coli strain A32

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AOY85_RS00840
• Protein ID: WP_000897302.1
• Coordinates: 175762..176073 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY85_RS00845
• Protein ID: WP_000356397.1
• Coordinates: 176074..176364 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY85_RS00810 (170792)	170792..171577	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
AOY85_RS00815 (171676)	171676..172275	+	600	WP_001314324.1	glucose-1-phosphatase	-
AOY85_RS00820 (172269)	172269..173141	+	873	WP_240727533.1	virulence factor BrkB family protein	-
AOY85_RS00825 (173138)	173138..173575	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY85_RS00830 (173620)	173620..174561	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY85_RS00835 (174625)	174625..175533	-	909	WP_001331553.1	alpha/beta hydrolase	-
AOY85_RS00840 (175762)	175762..176073	+	312	WP_000897302.1	hypothetical protein	Toxin
AOY85_RS00845 (176074)	176074..176364	+	291	WP_000356397.1	NadS family protein	Antitoxin
AOY85_RS00850 (176723)	176723..177001	+	279	WP_001296612.1	hypothetical protein	-
AOY85_RS00855 (177397)	177397..177615	+	219	WP_001314326.1	CopG family transcriptional regulator	-
AOY85_RS00860 (177831)	177831..178760	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
AOY85_RS00865 (178757)	178757..179392	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY85_RS00870 (179389)	179389..180291	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T103350 WP_000897302.1 NZ_CP028743:175762-176073 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103350 NZ_CP028743:175762-176073 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103350 WP_000356397.1 NZ_CP028743:176074-176364 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103350 NZ_CP028743:176074-176364 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416