Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2384435..2384988 Replicon chromosome
Accession NZ_CP028742
Organism Escherichia coli strain A33

Toxin (Protein)


Gene name higB Uniprot ID A0A0G9FUJ7
Locus tag AOY86_RS11490 Protein ID WP_000605680.1
Coordinates 2384719..2384988 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AOY86_RS11485 Protein ID WP_000781370.1
Coordinates 2384435..2384719 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY86_RS11470 (2379616) 2379616..2382663 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
AOY86_RS11475 (2382676) 2382676..2383560 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
AOY86_RS11480 (2383553) 2383553..2384206 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
AOY86_RS11485 (2384435) 2384435..2384719 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
AOY86_RS11490 (2384719) 2384719..2384988 - 270 WP_000605680.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY86_RS11495 (2385174) 2385174..2386184 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
AOY86_RS11500 (2386318) 2386318..2388015 - 1698 WP_000433464.1 malate dehydrogenase -
AOY86_RS11505 (2388172) 2388172..2388309 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AOY86_RS11510 (2388411) 2388411..2388626 - 216 WP_000495766.1 biofilm-dependent modulation protein -
AOY86_RS11515 (2388971) 2388971..2389402 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10181.73 Da        Isoelectric Point: 6.8582

>T103334 WP_000605680.1 NZ_CP028742:c2384988-2384719 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

>T103334 NZ_CP028742:c2384988-2384719 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACACGATT
ACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTACATCCGTTAA
GCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAATGGCGATGCA
TACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT103334 WP_000781370.1 NZ_CP028742:c2384719-2384435 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT103334 NZ_CP028742:c2384719-2384435 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FUJ7

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References