Detailed information of TA system TA103324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 172348..172950
• Replicon: chromosome
• Accession: NZ_CP028742
• Organism: Escherichia coli strain A33

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AOY86_RS00830
• Protein ID: WP_000897302.1
• Coordinates: 172348..172659 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY86_RS00835
• Protein ID: WP_000356397.1
• Coordinates: 172660..172950 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY86_RS00805 (168262)	168262..168861	+	600	WP_001314324.1	glucose-1-phosphatase	-
AOY86_RS00810 (168855)	168855..169727	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY86_RS00815 (169724)	169724..170161	+	438	WP_000560990.1	D-aminoacyl-tRNA deacylase	-
AOY86_RS00820 (170206)	170206..171147	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY86_RS00825 (171211)	171211..172119	-	909	WP_001331553.1	alpha/beta hydrolase	-
AOY86_RS00830 (172348)	172348..172659	+	312	WP_000897302.1	hypothetical protein	Toxin
AOY86_RS00835 (172660)	172660..172950	+	291	WP_000356397.1	NadS family protein	Antitoxin
AOY86_RS00840 (173309)	173309..173587	+	279	WP_001296612.1	hypothetical protein	-
AOY86_RS00845 (173983)	173983..174201	+	219	WP_001314326.1	CopG family transcriptional regulator	-
AOY86_RS00850 (174417)	174417..175346	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
AOY86_RS00855 (175343)	175343..175978	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY86_RS00860 (175975)	175975..176877	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T103324 WP_000897302.1 NZ_CP028742:172348-172659 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103324 NZ_CP028742:172348-172659 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103324 WP_000356397.1 NZ_CP028742:172660-172950 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103324 NZ_CP028742:172660-172950 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416