Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4244794..4245521 Replicon chromosome
Accession NZ_CP028741
Organism Escherichia coli strain A34

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag AOY87_RS20880 Protein ID WP_000550189.1
Coordinates 4245207..4245521 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag AOY87_RS20875 Protein ID WP_000560266.1
Coordinates 4244794..4245210 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY87_RS20865 (4239954) 4239954..4242305 + 2352 WP_240765678.1 alpha-glucosidase -
AOY87_RS20870 (4242731) 4242731..4244749 + 2019 WP_240765679.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
AOY87_RS20875 (4244794) 4244794..4245210 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
AOY87_RS20880 (4245207) 4245207..4245521 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
AOY87_RS20885 (4245806) 4245806..4246942 - 1137 WP_001514565.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
AOY87_RS20890 (4247027) 4247027..4247530 + 504 WP_240765680.1 M48 family metallopeptidase -
AOY87_RS20895 (4247607) 4247607..4248290 + 684 WP_001183040.1 vancomycin high temperature exclusion protein -
AOY87_RS20900 (4248369) 4248369..4249355 + 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T103321 WP_000550189.1 NZ_CP028741:c4245521-4245207 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T103321 NZ_CP028741:c4245521-4245207 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT103321 WP_000560266.1 NZ_CP028741:c4245210-4244794 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT103321 NZ_CP028741:c4245210-4244794 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCAAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References