Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1768379..1768881 Replicon chromosome
Accession NZ_CP028741
Organism Escherichia coli strain A34

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AOY87_RS08445 Protein ID WP_000767829.1
Coordinates 1768627..1768881 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID -
Locus tag AOY87_RS08440 Protein ID WP_096884122.1
Coordinates 1768379..1768630 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY87_RS08415 (1763556) 1763556..1764623 - 1068 WP_077039640.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AOY87_RS08420 (1764623) 1764623..1765693 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
AOY87_RS08425 (1765690) 1765690..1766994 - 1305 WP_240765925.1 histidinol dehydrogenase -
AOY87_RS08430 (1767000) 1767000..1767899 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
AOY87_RS08435 (1768045) 1768045..1768095 - 51 WP_001364200.1 his operon leader peptide -
AOY87_RS08440 (1768379) 1768379..1768630 + 252 WP_096884122.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AOY87_RS08445 (1768627) 1768627..1768881 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AOY87_RS08450 (1768964) 1768964..1769788 + 825 WP_000754758.1 SDR family oxidoreductase -
AOY87_RS08455 (1769834) 1769834..1770763 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
AOY87_RS08460 (1770978) 1770978..1771040 + 63 WP_010723108.1 membrane protein YoeI -
AOY87_RS08465 (1771030) 1771030..1772388 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AOY87_RS08470 (1772567) 1772567..1773625 + 1059 WP_000492305.1 thiosulfate utilization transporter TsuA/YeeE -
AOY87_RS08475 (1773639) 1773639..1773866 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T103308 WP_000767829.1 NZ_CP028741:1768627-1768881 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T103308 NZ_CP028741:1768627-1768881 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9322.55 Da        Isoelectric Point: 4.7954

>AT103308 WP_096884122.1 NZ_CP028741:1768379-1768630 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLQRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT103308 NZ_CP028741:1768379-1768630 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACAGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure

References