Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 960179..960720 Replicon chromosome
Accession NZ_CP028740
Organism Escherichia coli strain A35

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag AOY88_RS04495 Protein ID WP_000615976.1
Coordinates 960179..960457 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AOY88_RS04500 Protein ID WP_000729704.1
Coordinates 960460..960720 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY88_RS04480 (957761) 957761..958339 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AOY88_RS04485 (958545) 958545..959312 + 768 WP_000333380.1 class II glutamine amidotransferase -
AOY88_RS04490 (959283) 959283..960023 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY88_RS04495 (960179) 960179..960457 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY88_RS04500 (960460) 960460..960720 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY88_RS04505 (960906) 960906..961679 + 774 WP_000543897.1 C40 family peptidase -
AOY88_RS04510 (961736) 961736..962092 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
AOY88_RS04515 (962085) 962085..962363 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AOY88_RS04520 (962468) 962468..964561 - 2094 WP_001597626.1 flagellar biosynthesis protein FlhA -
AOY88_RS04525 (964545) 964545..965684 - 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T103281 WP_000615976.1 NZ_CP028740:c960457-960179 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103281 NZ_CP028740:c960457-960179 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT103281 WP_000729704.1 NZ_CP028740:c960720-960460 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103281 NZ_CP028740:c960720-960460 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References