Detailed information of TA system TA103278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 150525..151127
• Replicon: chromosome
• Accession: NZ_CP028740
• Organism: Escherichia coli strain A35

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY88_RS00740
• Protein ID: WP_000897305.1
• Coordinates: 150525..150836 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY88_RS00745
• Protein ID: WP_000356397.1
• Coordinates: 150837..151127 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY88_RS00715 (146439)	146439..147038	+	600	WP_001295269.1	glucose-1-phosphatase	-
AOY88_RS00720 (147032)	147032..147904	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY88_RS00725 (147901)	147901..148338	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY88_RS00730 (148383)	148383..149324	+	942	WP_001395735.1	fatty acid biosynthesis protein FabY	-
AOY88_RS00735 (149388)	149388..150296	-	909	WP_001162704.1	alpha/beta hydrolase	-
AOY88_RS00740 (150525)	150525..150836	+	312	WP_000897305.1	hypothetical protein	Toxin
AOY88_RS00745 (150837)	150837..151127	+	291	WP_000356397.1	NadS family protein	Antitoxin
AOY88_RS00750 (151732)	151732..151950	+	219	WP_001297075.1	CopG family transcriptional regulator	-
AOY88_RS00755 (152169)	152169..152411	+	243	WP_001086388.1	protein YiiF	-
AOY88_RS00760 (152741)	152741..153670	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AOY88_RS00765 (153667)	153667..154302	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY88_RS00770 (154299)	154299..155201	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103278 WP_000897305.1 NZ_CP028740:150525-150836 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103278 NZ_CP028740:150525-150836 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103278 WP_000356397.1 NZ_CP028740:150837-151127 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103278 NZ_CP028740:150837-151127 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7