Detailed information of TA system TA103276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4459513..4460312
• Replicon: chromosome
• Accession: NZ_CP028739
• Organism: Escherichia coli strain A36

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: AOY89_RS21680
• Protein ID: WP_000347273.1
• Coordinates: 4459848..4460312 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: AOY89_RS21675
• Protein ID: WP_001308975.1
• Coordinates: 4459513..4459848 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY89_RS21660 (4455296)	4455296..4456066	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
AOY89_RS21665 (4456059)	4456059..4457417	-	1359	WP_240745574.1	galactarate/glucarate/glycerate transporter GarP	-
AOY89_RS21670 (4457792)	4457792..4459364	+	1573	Protein_4243	galactarate dehydratase	-
AOY89_RS21675 (4459513)	4459513..4459848	+	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
AOY89_RS21680 (4459848)	4459848..4460312	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
AOY89_RS21685 (4460367)	4460367..4461176	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
AOY89_RS21690 (4461425)	4461425..4462705	+	1281	WP_000681958.1	tagatose-bisphosphate aldolase subunit KbaZ	-
AOY89_RS21695 (4462728)	4462728..4463201	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
AOY89_RS21700 (4463212)	4463212..4463991	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
AOY89_RS21705 (4463981)	4463981..4464859	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
AOY89_RS21710 (4464877)	4464877..4465311	+	435	WP_000948837.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T103276 WP_000347273.1 NZ_CP028739:4459848-4460312 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T103276 NZ_CP028739:4459848-4460312 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT103276 WP_001308975.1 NZ_CP028739:4459513-4459848 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT103276 NZ_CP028739:4459513-4459848 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08