Detailed information of TA system TA103261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1451630..1452357
• Replicon: chromosome
• Accession: NZ_CP028739
• Organism: Escherichia coli strain A36

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: AOY89_RS06940
• Protein ID: WP_000547563.1
• Coordinates: 1451630..1451941 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY89_RS06945
• Protein ID: WP_000126304.1
• Coordinates: 1451938..1452357 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY89_RS06910 (1446771)	1446771..1448480	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
AOY89_RS06915 (1448490)	1448490..1449032	+	543	WP_000493799.1	formate hydrogenlyase subunit HycF	-
AOY89_RS06920 (1449032)	1449032..1449799	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
AOY89_RS06925 (1449796)	1449796..1450206	+	411	WP_001291913.1	formate hydrogenlyase assembly protein HycH	-
AOY89_RS06930 (1450199)	1450199..1450669	+	471	WP_042024242.1	hydrogenase maturation peptidase HycI	-
AOY89_RS06935 (1450712)	1450712..1451467	+	756	WP_024262088.1	hypothetical protein	-
AOY89_RS06940 (1451630)	1451630..1451941	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
AOY89_RS06945 (1451938)	1451938..1452357	+	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
AOY89_RS06950 (1452449)	1452449..1452877	-	429	WP_000536064.1	hypothetical protein	-
AOY89_RS06955 (1453262)	1453262..1453789	+	528	WP_001078777.1	electron transport protein HydN	-
AOY89_RS06960 (1453942)	1453942..1456194	+	2253	WP_060588771.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T103261 WP_000547563.1 NZ_CP028739:1451630-1451941 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T103261 NZ_CP028739:1451630-1451941 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGCAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT103261 WP_000126304.1 NZ_CP028739:1451938-1452357 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT103261 NZ_CP028739:1451938-1452357 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22