Detailed information of TA system TA103254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 182062..182664
• Replicon: chromosome
• Accession: NZ_CP028739
• Organism: Escherichia coli strain A36

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AOY89_RS00895
• Protein ID: WP_000897302.1
• Coordinates: 182062..182373 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY89_RS00900
• Protein ID: WP_000356397.1
• Coordinates: 182374..182664 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY89_RS00865 (177092)	177092..177877	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
AOY89_RS00870 (177976)	177976..178575	+	600	WP_001298594.1	glucose-1-phosphatase	-
AOY89_RS00875 (178569)	178569..179441	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY89_RS00880 (179438)	179438..179875	+	438	WP_000560990.1	D-aminoacyl-tRNA deacylase	-
AOY89_RS00885 (179920)	179920..180861	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY89_RS00890 (180925)	180925..181833	-	909	WP_001331553.1	alpha/beta hydrolase	-
AOY89_RS00895 (182062)	182062..182373	+	312	WP_000897302.1	hypothetical protein	Toxin
AOY89_RS00900 (182374)	182374..182664	+	291	WP_000356397.1	NadS family protein	Antitoxin
AOY89_RS00905 (183023)	183023..183301	+	279	WP_001296612.1	hypothetical protein	-
AOY89_RS00910 (183697)	183697..183915	+	219	WP_001314326.1	CopG family transcriptional regulator	-
AOY89_RS00915 (184131)	184131..185060	-	930	WP_032359289.1	formate dehydrogenase accessory protein FdhE	-
AOY89_RS00920 (185057)	185057..185692	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY89_RS00925 (185689)	185689..186591	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T103254 WP_000897302.1 NZ_CP028739:182062-182373 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103254 NZ_CP028739:182062-182373 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103254 WP_000356397.1 NZ_CP028739:182374-182664 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103254 NZ_CP028739:182374-182664 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416