Detailed information of TA system TA103253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4704026..4704628
• Replicon: chromosome
• Accession: NZ_CP028738
• Organism: Escherichia coli strain A38

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY91_RS22785
• Protein ID: WP_000897305.1
• Coordinates: 4704317..4704628 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY91_RS22780
• Protein ID: WP_000356397.1
• Coordinates: 4704026..4704316 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY91_RS22755 (4699951)	4699951..4700853	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
AOY91_RS22760 (4700850)	4700850..4701485	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY91_RS22765 (4701482)	4701482..4702411	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AOY91_RS22770 (4702741)	4702741..4702983	-	243	WP_001087409.1	protein YiiF	-
AOY91_RS22775 (4703203)	4703203..4703421	-	219	WP_001315930.1	CopG family transcriptional regulator	-
AOY91_RS22780 (4704026)	4704026..4704316	-	291	WP_000356397.1	NadS family protein	Antitoxin
AOY91_RS22785 (4704317)	4704317..4704628	-	312	WP_000897305.1	hypothetical protein	Toxin
AOY91_RS22790 (4704857)	4704857..4705765	+	909	WP_001162704.1	alpha/beta hydrolase	-
AOY91_RS22795 (4705829)	4705829..4706770	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY91_RS22800 (4706815)	4706815..4707252	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY91_RS22805 (4707249)	4707249..4708121	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY91_RS22810 (4708115)	4708115..4708714	-	600	WP_001295269.1	glucose-1-phosphatase	-
AOY91_RS22815 (4708813)	4708813..4709598	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103253 WP_000897305.1 NZ_CP028738:c4704628-4704317 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103253 NZ_CP028738:c4704628-4704317 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103253 WP_000356397.1 NZ_CP028738:c4704316-4704026 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103253 NZ_CP028738:c4704316-4704026 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7