103249

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3844213..3844754 Replicon chromosome
Accession NZ_CP028738
Organism Escherichia coli strain A38

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag AOY91_RS18775 Protein ID WP_000615976.1
Coordinates 3844476..3844754 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AOY91_RS18770 Protein ID WP_000729704.1
Coordinates 3844213..3844473 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY91_RS18745 (3839996) 3839996..3840781 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
AOY91_RS18750 (3840753) 3840753..3842465 + 1713 Protein_3683 flagellar biosynthesis protein FlhA -
AOY91_RS18755 (3842570) 3842570..3842848 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AOY91_RS18760 (3842841) 3842841..3843197 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
AOY91_RS18765 (3843254) 3843254..3844027 - 774 WP_000543895.1 C40 family peptidase -
AOY91_RS18770 (3844213) 3844213..3844473 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY91_RS18775 (3844476) 3844476..3844754 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY91_RS18780 (3844910) 3844910..3845650 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY91_RS18785 (3845621) 3845621..3846388 - 768 WP_000333380.1 class II glutamine amidotransferase -
AOY91_RS18790 (3846594) 3846594..3847172 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3842841..3855540 12699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T103249 WP_000615976.1 NZ_CP028738:3844476-3844754 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103249 NZ_CP028738:3844476-3844754 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT103249 WP_000729704.1 NZ_CP028738:3844213-3844473 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103249 NZ_CP028738:3844213-3844473 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6Z6

References