103230

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 651021..651820 Replicon chromosome
Accession NZ_CP028738
Organism Escherichia coli strain A38

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag AOY91_RS03170 Protein ID WP_000347266.1
Coordinates 651021..651485 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag AOY91_RS03175 Protein ID WP_001307405.1
Coordinates 651485..651820 (-) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY91_RS03140 (646022) 646022..646456 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
AOY91_RS03145 (646474) 646474..647352 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
AOY91_RS03150 (647342) 647342..648121 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
AOY91_RS03155 (648132) 648132..648605 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
AOY91_RS03160 (648628) 648628..649908 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
AOY91_RS03165 (650157) 650157..650966 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
AOY91_RS03170 (651021) 651021..651485 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
AOY91_RS03175 (651485) 651485..651820 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
AOY91_RS03180 (651969) 651969..653540 - 1572 WP_001273741.1 galactarate dehydratase -
AOY91_RS03185 (653915) 653915..655249 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
AOY91_RS03190 (655265) 655265..656035 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 651021..662808 11787


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T103230 WP_000347266.1 NZ_CP028738:c651485-651021 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T103230 NZ_CP028738:c651485-651021 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT103230 WP_001307405.1 NZ_CP028738:c651820-651485 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT103230 NZ_CP028738:c651820-651485 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A836NGD2


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References