Detailed information of TA system TA103227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4588916..4589518
• Replicon: chromosome
• Accession: NZ_CP028737
• Organism: Escherichia coli strain A39

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY92_RS22040
• Protein ID: WP_000897305.1
• Coordinates: 4589207..4589518 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY92_RS22035
• Protein ID: WP_000356397.1
• Coordinates: 4588916..4589206 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY92_RS22010 (4584843)	4584843..4585745	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
AOY92_RS22015 (4585742)	4585742..4586377	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY92_RS22020 (4586374)	4586374..4587303	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AOY92_RS22025 (4587633)	4587633..4587875	-	243	WP_001086388.1	protein YiiF	-
AOY92_RS22030 (4588094)	4588094..4588306	-	213	WP_077252770.1	CopG family transcriptional regulator	-
AOY92_RS22035 (4588916)	4588916..4589206	-	291	WP_000356397.1	NadS family protein	Antitoxin
AOY92_RS22040 (4589207)	4589207..4589518	-	312	WP_000897305.1	hypothetical protein	Toxin
AOY92_RS22045 (4589747)	4589747..4590655	+	909	WP_048963532.1	alpha/beta hydrolase	-
AOY92_RS22050 (4590719)	4590719..4591660	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
AOY92_RS22055 (4591705)	4591705..4592142	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY92_RS22060 (4592139)	4592139..4593011	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY92_RS22065 (4593005)	4593005..4593604	-	600	WP_001295269.1	glucose-1-phosphatase	-
AOY92_RS22070 (4593703)	4593703..4594488	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103227 WP_000897305.1 NZ_CP028737:c4589518-4589207 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103227 NZ_CP028737:c4589518-4589207 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103227 WP_000356397.1 NZ_CP028737:c4589206-4588916 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103227 NZ_CP028737:c4589206-4588916 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7