Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3677242..3677783 Replicon chromosome
Accession NZ_CP028737
Organism Escherichia coli strain A39

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag AOY92_RS17760 Protein ID WP_000615976.1
Coordinates 3677505..3677783 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AOY92_RS17755 Protein ID WP_000729704.1
Coordinates 3677242..3677502 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY92_RS17730 (3673025) 3673025..3673810 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
AOY92_RS17735 (3673782) 3673782..3675494 + 1713 Protein_3476 flagellar biosynthesis protein FlhA -
AOY92_RS17740 (3675599) 3675599..3675877 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AOY92_RS17745 (3675870) 3675870..3676226 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
AOY92_RS17750 (3676283) 3676283..3677056 - 774 WP_000543899.1 C40 family peptidase -
AOY92_RS17755 (3677242) 3677242..3677502 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY92_RS17760 (3677505) 3677505..3677783 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY92_RS17765 (3677939) 3677939..3678679 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY92_RS17770 (3678650) 3678650..3679417 - 768 WP_000333380.1 class II glutamine amidotransferase -
AOY92_RS17775 (3679623) 3679623..3680201 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T103223 WP_000615976.1 NZ_CP028737:3677505-3677783 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103223 NZ_CP028737:3677505-3677783 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT103223 WP_000729704.1 NZ_CP028737:3677242-3677502 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103223 NZ_CP028737:3677242-3677502 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References